Single Molecule TIRF Microscopy on Nanostructured Surfaces
Single molecule microscopy uses total internal reflection excitation to allow the tracking of fluorescent molecules along surfaces. The great advantage of this technique is the possibility to track individual molecules as opposed to ensemble measurements. The behaviour of single molecules defines whether a cell lives, dies, or responds to a specific mechanical or drug treatment. Single molecule microscopy has begun to reveal the number, the spatial dynamics, and the functionalities of molecules outside and inside living cells.