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Self-Assembled Phage-Based Colloids for High Localized Enzymatic Activity

2019

Article

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Catalytically active colloids are model systems for chemical motors and active matter. It is desirable to replace the inorganic catalysts and the toxic fuels that are often used, with biocompatible enzymatic reactions. However, compared to inorganic catalysts, enzyme-coated colloids tend to exhibit less activity. Here, we show that the self-assembly of genetically engineered M13 bacteriophages that bind enzymes to magnetic beads ensures high and localized enzymatic activity. These phage-decorated colloids provide a proteinaceous environment for directed enzyme immobilization. The magnetic properties of the colloidal carrier particle permit repeated enzyme recovery from a reaction solution, while the enzymatic activity is retained. Moreover, localizing the phage-based construct with a magnetic field in a microcontainer allows the enzyme-phage-colloids to function as an enzymatic micropump, where the enzymatic reaction generates a fluid flow. This system shows the fastest fluid flow reported to date by a biocompatible enzymatic micropump. In addition, it is functional in complex media including blood where the enzyme driven micropump can be powered at the physiological blood-urea concentration.

Author(s): Alarcon-Correa, Mariana and Guenther, Jan-Philipp and Troll, Jonas and Kadiri, Vincent Mauricio and Bill, Joachim and Fischer, Peer and Rothenstein, Dirk
Journal: ACS Nano
Year: 2019
Month: March
Day: 28

Department(s): Micro, Nano, and Molecular Systems
Bibtex Type: Article (article)
Paper Type: Journal

DOI: 10.1021/acsnano.9b0140
URL: https://pubs.acs.org/doi/pdf/10.1021/acsnano.9b01408

BibTex

@article{2019Alarcon,
  title = {Self-Assembled Phage-Based Colloids for High Localized Enzymatic Activity},
  author = {Alarcon-Correa, Mariana and Guenther, Jan-Philipp and Troll, Jonas and Kadiri, Vincent Mauricio and Bill, Joachim and Fischer, Peer and Rothenstein, Dirk},
  journal = {ACS Nano},
  month = mar,
  year = {2019},
  url = {https://pubs.acs.org/doi/pdf/10.1021/acsnano.9b01408},
  month_numeric = {3}
}