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Influence of Different Assignment Conditions on the Determination of Symmetric Homo-dimeric Structures with ARIA

2009

Article

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The ambiguous restraint for iterative assignment (ARIA) approach for NMR structure calculation is evaluated for symmetric homodimeric proteins by assessing the effect of several data analysis and assignment methods on the structure quality. In particular, we study the effects of network anchoring and spin-diffusion correction. The spin-diffusion correction improves the protein structure quality systematically, whereas network anchoring enhances the assignment efficiency by speeding up the convergence and coping with highly ambiguous data. For some homodimeric folds, network anchoring has been proved essential for unraveling both chain and proton assignment ambiguities.

Author(s): Bardiaux, B. and Bernard, A. and Rieping, W. and Habeck, M. and Malliavin, TE. and Nilges, M.
Journal: Proteins
Volume: 75
Number (issue): 3
Pages: 569-585
Year: 2009
Month: May
Day: 0

Department(s): Empirical Inference
Bibtex Type: Article (article)

Digital: 0
DOI: 10.1002/prot.22268
Language: en
Organization: Max-Planck-Gesellschaft
School: Biologische Kybernetik

Links: Web

BibTex

@article{5384,
  title = {Influence of Different Assignment Conditions on the Determination of Symmetric Homo-dimeric Structures with ARIA},
  author = {Bardiaux, B. and Bernard, A. and Rieping, W. and Habeck, M. and Malliavin, TE. and Nilges, M.},
  journal = {Proteins},
  volume = {75},
  number = {3},
  pages = {569-585},
  organization = {Max-Planck-Gesellschaft},
  school = {Biologische Kybernetik},
  month = may,
  year = {2009},
  month_numeric = {5}
}