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HPLC analysis and pharmacokinetic study of quercitrin and isoquercitrin in rat plasma after administration of Hypericum japonicum thunb. extract.

2007

Article

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A simple HPLC method was developed for determination of quercitrin and isoquercitrin in rat plasma. Reversed-phase HPLC was employed for the quantitative analysis using kaempferol-3-O--d-glucopyranoside-7-O--l-rhamnoside as an internal standard. Following extraction from the plasma samples with ethyl acetate-isopropanol (95:5, v/v), these two compounds were successfully separated on a Luna C18 column (250 × 4.6 mm, 5 µm) with isocratic elution of acetonitrile-0.5% aqueous acetic acid (17:83, v/v) as the mobile phase. The flow-rate was set at 1 mL/min and the eluent was detected at 350 nm for both quercitrin and isoquercitrin. The method was linear over the studied ranges of 50-6000 and 50-5000 ng/mL for quercitrin and isoquercitrin, respectively. The intra- and inter-day precisions of the analysis were better than 13.1 and 13.2%, respectively. The lower limits of quantitation for quercitrin and isoquercitrin in plasma were both of 50 ng/mL. The mean extraction recoveries were 73 and 61% for quercitrin and i soquercitrin, respectively. The validated method was successfully applied to pharmacokinetic studies of the two analytes in rat plasma after the oral administration of Hypericum japonicum thunb. ethanol extract.

Author(s): Li, J. and Wang, WZ. and Zhang, L. and Chen, HX. and Bi, SK.
Journal: Biomedical Chromatography
Volume: 22
Number (issue): 4
Pages: 374-378
Year: 2007
Month: December
Day: 5

Department(s): Empirical Inference
Bibtex Type: Article (article)

Digital: 0
DOI: 10.1002/bmc.942
Language: en
Organization: Max-Planck-Gesellschaft
School: Biologische Kybernetik

Links: Web

BibTex

@article{6169,
  title = {HPLC analysis and pharmacokinetic study of quercitrin and isoquercitrin in rat plasma after administration of Hypericum japonicum thunb. extract.},
  author = {Li, J. and Wang, WZ. and Zhang, L. and Chen, HX. and Bi, SK.},
  journal = {Biomedical Chromatography},
  volume = {22},
  number = {4},
  pages = {374-378},
  organization = {Max-Planck-Gesellschaft},
  school = {Biologische Kybernetik},
  month = dec,
  year = {2007},
  month_numeric = {12}
}