Kristen Kozielski

Physical Intelligence

My doctoral background was in polymeric nanoparticles for gene delivery, with several medical applications including brain cancer. My current work is on a reversibly self-assembled microswimmer that responds to pH, as well as multiferroic nanoparticles for wireless deep brain stimulation. Overall, my interests are in materials with applications in minimally-invasive medicine.

Ph.D. in Biomedical Engineering, Johns Hopkins University, 2016

B.S. in Biomedical Engineering, Johns Hopkins University, 2010

5 results (View BibTeX file of all listed publications)

2020

Injectable Nanoelectrodes Enable Wireless Deep Brain Stimulation of Native Tissue in Freely Moving Mice

Kozielski, K. L., Jahanshahi, A., Gilbert, H. B., Yu, Y., Erin, O., Francisco, D., Alosaimi, F., Temel, Y., Sitti, M.

bioRxiv, Cold Spring Harbor Laboratory, 2020 (article)

[BibTex]

2020

pi Kozielski, K. L., Jahanshahi, A., Gilbert, H. B., Yu, Y., Erin, O., Francisco, D., Alosaimi, F., Temel, Y., Sitti, M. Injectable Nanoelectrodes Enable Wireless Deep Brain Stimulation of Native Tissue in Freely Moving Mice bioRxiv, Cold Spring Harbor Laboratory, 2020 (article)

[BibTex]

2019

Cancer-selective nanoparticles for combinatorial siRNA delivery to primary human GBM in vitro and in vivo

Kozielski, K. L., Ruiz-Valls, A., Tzeng, S. Y., Guerrero-Cázares, H., Rui, Y., Li, Y., Vaughan, H. J., Gionet-Gonzales, M., Vantucci, C., Kim, J., others,

Biomaterials, 209, Elsevier, 2019 (article)

DOI [BibTex]

2019

pi Kozielski, K. L., Ruiz-Valls, A., Tzeng, S. Y., Guerrero-Cázares, H., Rui, Y., Li, Y., Vaughan, H. J., Gionet-Gonzales, M., Vantucci, C., Kim, J., others, Cancer-selective nanoparticles for combinatorial siRNA delivery to primary human GBM in vitro and in vivo Biomaterials, 209, Elsevier, 2019 (article)

DOI [BibTex]

2018

Redox metals homeostasis in multiple sclerosis and amyotrophic lateral sclerosis: a review

Sheykhansari, S., Kozielski, K., Bill, J., Sitti, M., Gemmati, D., Zamboni, P., Singh, A. V.

Cell Death \& Disease, 9(3):348, March 2018 (article)

Abstract

The effect of redox metals such as iron and copper on multiple sclerosis and amyotrophic lateral sclerosis has been intensively studied. However, the origin of these disorders remains uncertain. This review article critically describes the physiology of redox metals that produce oxidative stress, which in turn leads to cascades of immunomodulatory alteration of neurons in multiple sclerosis and amyotrophic lateral sclerosis. Iron and copper overload has been well established in motor neurons of these diseases' lesions. On the other hand, the role of other metals like cadmium participating indirectly in the redox cascade of neurobiological mechanism is less studied. In the second part of this review, we focus on this less conspicuous correlation between cadmium as an inactive-redox metal and multiple sclerosis and amyotrophic lateral sclerosis, providing novel treatment modalities and approaches as future prospects.

link (url) DOI [BibTex]

2018

pi Sheykhansari, S., Kozielski, K., Bill, J., Sitti, M., Gemmati, D., Zamboni, P., Singh, A. V. Redox metals homeostasis in multiple sclerosis and amyotrophic lateral sclerosis: a review Cell Death \& Disease, 9(3):348, March 2018 (article)

link (url) DOI [BibTex]

2017

Gene delivery particle engineering strategies for shape-dependent targeting of cells and tissues.

Kozielski, K., Sitti, M.

Current Gene Therapy, 17, 2017 (article)

Abstract

Background: Successful gene delivery requires overcoming both systemic and intracellular obstacles before the nucleic acid cargo can successfully reach its tissue and subcellular target location. Materials & Methods: Non-viral mechanisms to enable targeting while avoiding off-target delivery have arisen via biological, chemical, and physical engineering strategies. Discussion: Herein we will discuss the physical parameters in particle design that promote tissue- and cell-targeted delivery of genetic cargo. We will discuss systemic concerns, such as circulation, tissue localization, and clearance, as well as cell-scale obstacles, such as cellular uptake and nucleic acid packaging. Conclusion: In particular, we will focus on engineering particle shape and size in order to enhance delivery and promote precise targeting. We will also address methods to program or change particle shape in situ using environmentally triggered cues.

DOI [BibTex]

2017

pi Kozielski, K., Sitti, M. Gene delivery particle engineering strategies for shape-dependent targeting of cells and tissues. Current Gene Therapy, 17, 2017 (article)

DOI [BibTex]

Mobile microrobots for bioengineering applications

Ceylan, H., Giltinan, J., Kozielski, K., Sitti, M.

Lab on a Chip, 17(10):1705-1724, Royal Society of Chemistry, 2017 (article)

Abstract

Untethered micron-scale mobile robots can navigate and non-invasively perform specific tasks inside unprecedented and hard-to-reach inner human body sites and inside enclosed organ-on-a-chip microfluidic devices with live cells. They are aimed to operate robustly and safely in complex physiological environments where they will have a transforming impact in bioengineering and healthcare. Research along this line has already demonstrated significant progress, increasing attention, and high promise over the past several years. The first-generation microrobots, which could deliver therapeutics and other cargo to targeted specific body sites, have just been started to be tested inside small animals toward clinical use. Here, we review frontline advances in design, fabrication, and testing of untethered mobile microrobots for bioengineering applications. We convey the most impactful and recent strategies in actuation, mobility, sensing, and other functional capabilities of mobile microrobots, and discuss their potential advantages and drawbacks to operate inside complex, enclosed and physiologically relevant environments. We lastly draw an outlook to provide directions in the veins of more sophisticated designs and applications, considering biodegradability, immunogenicity, mobility, sensing, and possible medical interventions in complex microenvironments.

DOI [BibTex]

pi Ceylan, H., Giltinan, J., Kozielski, K., Sitti, M. Mobile microrobots for bioengineering applications Lab on a Chip, 17(10):1705-1724, Royal Society of Chemistry, 2017 (article)

DOI [BibTex]

Kristen Kozielski

2020

Injectable Nanoelectrodes Enable Wireless Deep Brain Stimulation of Native Tissue in Freely Moving Mice

2020

2019

Cancer-selective nanoparticles for combinatorial siRNA delivery to primary human GBM in vitro and in vivo

2019

2018

Redox metals homeostasis in multiple sclerosis and amyotrophic lateral sclerosis: a review

2018

2017

Gene delivery particle engineering strategies for shape-dependent targeting of cells and tissues.

2017

Mobile microrobots for bioengineering applications

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